After whole-mount nuclear (all cells) and fast-green (FG) staining, an E15.5 embryo was cleared & imaged with light-sheet microscopy by C. Langrez. Only nuclear staining is shown here and the final 3D image was optimised for surface visualisation by S. Zakany. Internal structures are visible here (nuclear staining) and here (FG staining).
Check here a flight-through across the whole 3D image.
We introduced in 2023 a simple and robust method of whole-mount staining with the ‘Fast Green’ dye that provides unmatched visualisation of 3D collagen network architecture, especially useful in developmental and cancer biology
The scientific publication & additional images are available here.