Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the ‘Fast Green’ dye that provides unmatched visualisation of collagen 3D network architecture, via confocal or light- sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.
High-Resolution Confocal and Light-Sheet Imaging of Collagen 3D Network Architecture in Very Large Samples
Timin Grigorii & Michel C. Milinkovitch
iSciences 26, 106452 (2023)
Correspondence: Michel C. Milinkovitch
Use the photo gallery below to download the full-resolution images used in the various panels of Figures 1 to 7.