Fast-Green Staining of Collagen 3D Network

A revolutionary method for imaging collagen 3D network architecture in large samples with unmatched high-resolution

Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the ‘Fast Green’ dye that provides visualisation of collagen 3D network architecture, via confocal or light-sheet microscopy, with unmatched resolution. This new approach is compatible with tissue clearing techniques and immunostaining.