R²OBBIE-3D

Phenotypes at larger scales (i.e., larger than those probed with LSFM and confocal microscopy) remain poorly amenable to quantitative analyses. To partially resolve that problem, we have developed R2OBBIE-3D, an integrated system that combines a robotic arm, a high-resolution digital colour camera, an illumination basket of high-intensity light-emitting diodes, and state-of-the-art 3D-reconstruction approaches. R2OBBIE generates accurate 3D models of biological objects between 1 and 100 cm, integrating colour-texture and geometry with a resolution of around 15 to 40 μm without the use of magnifying lenses! We extensively use R2OBBIE to study the dynamics of skin colour patterning in lizards and snakes (e.g., Nature 2017; Nature Communications 2021; Physical Review Letters 2022; Current Biology 2022).

• R2OBBIE-3D, a Fast Robotic High-Resolution System for Quantitative Phenotyping of Surface Geometry and Colour-Texture
  Martins A., Bessant M., Manukyan L., M.C. Milinkovitch
  PLOS ONE 10, 6 : e0126740 (2015)