After whole-mount nuclear (all cells) and fast-green (FG) staining, an E15.5 embryo was cleared & imaged with light-sheet microscopy by Carine Langrez. Only nuclear staining is shown here and the final 3D image was optimised for visualising calcified bones, collagen-rich tendons and large blood vessels. It was obtained with an in-house signal processing method —developed by Szabolcs Zakany and similar to ‘High Dynamic Range’— that leverages the 12-bit data produced with light-sheet microscopy.
Annotations give access to zoomed regions in the volumetric data.
Check here a movie of a flight-through across the whole 3D image but optimised for surface visualisation.
Scientific context
We introduced in 2023 a simple and robust method of whole-mount staining with the ‘Fast Green’ dye that provides unmatched visualisation of 3D collagen network architecture, especially useful in developmental and cancer biology.
The scientific publication & additional images are available here.