After whole-mount nuclear (all cells) and fast-green (FG) staining, an E15.5 embryo was cleared & imaged with light-sheet microscopy by C. Langrez. The final 3D image was obtained with an in-house signal processing method —similar to ‘High Dynamic Range’ and developed by S. Zakany— that leverages the 12-bit data produced with light-sheet microscopy. Only FG signal is shown here. Highest signal is visible in calcified bones and collagen-rich tendons and large blood vessels.
Check here a movie of a flight-through across the whole 3D image.
Annotations give access to zoomed regions in the volumetric data.
We introduced in 2023 a simple and robust method of whole-mount staining with the ‘Fast Green’ dye that provides unmatched visualisation of 3D collagen network architecture, especially useful in developmental and cancer biology.
The scientific publication & additional images are available here.